Mouse Cajal-Retzius cells’histogenesis and expression of the cell cycle-related proteins

doi:10.3969/j.issn.0529-1356.2013.01.002

Abstract

Abstract:

Objective To study Cajal-Retzius (CR) cells’ histogenesis and their expressions of cell cycle-related proteins in mouse. Methods Total 83 mice were used. Immunofluorescent labeling and BrdU assay were carried out at various embryonic and postnatal ages. Results 1. The birthday of CR cells was at about embryonic day 10 (E10) or 11 (E11). CR cells mainly distributed in the molecular layer of neocortex and hippocampus. Their number decreased with age increasing. 2.From E15 to adult, Cyclin A2, Cyclin E1, and CDT1 were expressed continuously in CR cells, but there was no any expression of Cyclin D1 in CR cells. Conclusion CR cells are important in the brain development. The decrease of CR cells with development is correlative with the activity of the neocortical development. CR cells in neocortex have been probably exited out cell cycle and steped in the G₀phase. If the CR cells split again, they will apoptosis because of their axons can not absorb and reversetransport the neurotrophic factors.

Key words: Cerebral cortex, development, Cajal-Retzius cells, immunocytochemistry, mouse

JIN Hai-xiao,FU Xing,KONG Wei-fang,MA Zhan-you, GAO Wen-jing, DENG Jin-bo*. Mouse Cajal-Retzius cells’histogenesis and expression of the cell cycle-related proteins[J]. AAS, 2013, 44(1 ): 6-12.

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